While the League of Legends fan canon has had a lot of champions that people believe may be queer, Neeko, the latest addition to the game, has been confirmed as lesbian by senior narrative writer Matt Dunn, who worked on the character. This makes her the first openly confirmed LGBTQ champion in the game.
Her voice interactions with other female champions fit in line with this, too. She’s very clearly attracted to the women of the game, asking Shyvana out on a date or commenting about how Evelynn makes her feel curious. Her lines towards men tend to be fairly hostile. She notes that they smell bad or that she doesn’t like them, with the exception of Ezreal, who she describes as, “Pretty! For a boy.”
Though many people believe Varus actually got the role of “first LGBTQ champion,” if you look at the lore, he’s more the result of an LGBTQ story rather than an LGBTQ character. Varus made up of two male partners and his weird Darkin-bow-demon-thing to make a new entity. While he’s an example of LGBTQ representation in the game, it’s unknown if Varus himself would be considered queer.
Ezreal, Vi and Fiora are also champions that fans believe to be queer in some form, whether they’re gay, lesbian, bisexual or pansexual, but there has never been any official confirmation of this from Riot Games.